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WGA3
Sigma
GenomePlex® WGA Reamplification Kit
Description
| General description | GenomePlex WGA Reamplification Kit utilizes a proprietary amplification method that is based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers. |
| Other Notes | The sequences of the universal primers provided in this kit are considered proprietary. |
| Features and Benefits | • Successfully produces a negative control sample without product • Maintains representation of the entire genome through subsequent reamplifications • Preserve previous source material by amplifying nanogram amounts of starting genomic DNA into microgram yields (on average up to 500-fold) in less than three hours |
| Legal Information | GenomePlex is a registered trademark of Rubicon Genomics, Inc. |
| Roche Enzyme Products Conveying Polymerase Patent RightsUse of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim (such as the patented 5′ Nuclease Process claims in US Patents Nos. 5,210,015 and 5,487,972), no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. |
Properties
| shipped in | wet ice |
| storage temp. | −20°C |
Components
| Kit component only | S A5606 10X Amplification Master Mix |
| Standard component | Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent 0.2 mL D7295 |
Related Products
| Related product | Z719218, Whole Genome Amplification: Methods Express Series |
References
| reference | Barker, D. L., et al., Two methods for whole-genome amplification enable accurate genotyping across a 2320-SNP linkage panel Genome Res. 14, 901-907, (2004) Abstract |
| Gribble, S., et al., Chromosome paints from single copies of chromosomes Chromosome Res. 12, 143-151, (2004) Abstract | |
| Thorstenson, Y. R. et al., An Automated Hydrodynamic Process for Controlled, Unbiased DNA Shearing Genome Res. 8, 848-855, (1998) Abstract | |
| Park, J. W., et al., Comparing Whole-Genome Amplification Methods and Sources for Biological Samples for Single-Nucleotide Polymorphism Genotyping Clin. Chem. 51(8), 1520-1523, (2004) |
